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JHC exPRESS: First Published October 31, 2005. doi:10.1369/jhc.5B6726.2005
Copyright © Histochemical Society, Inc.

A more recent version of this article appeared on March 1, 2006.
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Brief Reports

The Effect of Fixation to the Degradation of Nuclear and Mitochondrial DNA in Different Tissues

Franziska Miething 1*, Sandra Hering 1, Bärbel Hanschke 1 and Jan Dressler 1

1 Institute of Legal Medicine, Faculty of Medicine, University of Dresden Dresden, Germany

* To whom correspondence should be addressed. E-mail: irm{at}rcs.urz.tu-dresden.de.

Submitted on April 26, 2005
Accepted on 11 October 2005


  Abstract
Samples of different tissues were preserved in seven fixatives for periods of time extending from one to 336 days, to find out which fixatives reduce the time-dependent degradation of DNA and preserve the histological structure. To achieve these results three PCR systems were used: FGA and TC11 (both for nuclear DNA), and HV1 (for mt-DNA). For long-term storage in combination with amplification of nuclear and mt-DNA, consistent results were obtained in Carnoy’s solution and glutaraldehyde. Variable results were observed for buffered formalin; an mt-DNA product could be detected even after 3 months of fixation. As regards comparison of the different tissues, the quantities recovered from skeletal muscles and kidneys were higher than from the others.

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