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JHC exPRESS: First Published June 27, 2005. doi:10.1369/jhc.5C6659.2005
Copyright © Histochemical Society, Inc.

A more recent version of this article appeared on October 1, 2005.
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Rapid Communications

Resin Tissue Microarrays: a Universal Format for Immunohistochemistry

William J. Howat 1*, Anthony Warford 1, Joanne N. Mitchell 1, Kay F. Clarke 1, Jen S. Conquer 1 and John McCafferty 1

1 Atlas of Protein Expression Project, Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridgeshire, United Kingdom

* To whom correspondence should be addressed. E-mail: wjh{at}sanger.ac.uk.

Accepted on 16 May 2005


  Abstract
Tissue microarray (TMA) technology allows the miniaturization and characterisation of multiple tissue samples on a single slide and commonly uses formalin-fixed paraffin embedded (FFPE) tissue or acetone-fixed frozen tissue. The former provides good morphology but can compromise antigenicity whereas the latter provides compromised morphology with good antigenicity. Here, we report the development of TMA’s in glycol methacrylate resin which combine the advantages of both methods in one embedding format. Freshly collected tissue fixed in -20°C acetone or 10% neutral buffered formaldehyde were cored and arrayed into an intermediary medium of 2% agarose, before infiltration of the agarose array with glycol methacrylate resin. Acetone-fixed resin TMA demonstrated improved morphology over acetone-fixed frozen TMA with no loss of antigenicity. Staining for extracellular, cell surface and nuclear antigens could be realized with monoclonal and polyclonal antibodies as well as with monomeric single chain Fv preparations. In addition, when compared with FFPE TMA, formalin-fixed tissue in a resin TMA gave enhanced morphology and sub-cellular detail. Therefore, resin provides a universal format for the construction of tissue microarrays providing improved tissue morphology while retaining antigenicity, allows thin section preparation and could be used to replace preparation of frozen and FFPE TMA’s for freshly collected tissue.

Key Words: tissue microarray, resin TMA, glycol methacrylate, immunohistochemistry, single chain Fv


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