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JHC exPRESS: First Published July 11, 2005. doi:10.1369/jhc.5C6703.2005
Copyright © Histochemical Society, Inc.

A more recent version of this article appeared on October 1, 2005.
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Rapid Communications

An Alternative Pre-treatment Procedure in Animal Transmissible Spongiform Encephalopathies Diagnosis Using PrPsc Immunohistochemistry

Anna A. Bencsik 1*, Sabine O.S. Debeer 1 and Thierry G.M. Baron 1

1 AFSSA, Laboratoire d’Etudes et de Recherches en Pathologie Bovine et Hygiène des Viandes, Unité ATNC, Lyon, France

* To whom correspondence should be addressed. E-mail: a.bencsik{at}lyon.afssa.fr.

Submitted on March 30, 2005
Accepted on 7 June 2005


  Abstract
Because of its sensitivity, immunohistochemistry of abnormal prion protein (PrPsc) is more and more used in the diagnosis of transmissible spongiform encephalopathies (TSEs), such as scrapie and bovine spongiform encephalopathy (BSE). PrPsc immunohistochemistry requires a combination of pretreatments (chemical, heating and enzymatic), that way of application may depend on the anti-prion antibody considered. If these pretreatments are efficient for diagnosis purpose it may however be interesting to use an alternative way to detect efficiently PrPsc immunohistochemically using chemical pretreatments solely. Here we describe such pretreatments reporting the difficulty (section adhesion) but also the potential advantages of such methods (easy, quick, cheap and amplifying effect).

Key Words: BSE, diagnosis, antigen retrieval, immunohistochemistry, prion, scrapie


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A. A. Bencsik, A. W. Coleman, S. O.S. Debeer, H. Perron, and A. Moussa
Amplified Immunohistochemical Detection of PrPsc in Animal Transmissible Spongiform Encephalopathies Using Streptomycin
J. Histochem. Cytochem., August 1, 2006; 54(8): 849 - 853.
[Abstract] [Full Text] [PDF]



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