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JHC exPRESS: First Published August 22, 2005. doi:10.1369/jhc.5C6782.2005
Copyright © Histochemical Society, Inc.

A more recent version of this article appeared on February 1, 2006.
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Rapid Communications

Immunolocalization of ACE2 and AT2 Receptors in Rabbit Atherosclerotic Plaques

Anthony Zulli 1*, Louise M Burrell 1, Robert E. Widdop 1, M Jane Black 1, Brian F. Buxton 1 and David L. Hare 1

1 Departments of Cardiology (AZ,DLH), Cardiac Surgery (BFB), and Medicine (AZ,LMB), University of Melbourne, Austin Health, Heidelberg, Australia, and Department of Pharmacology (REW) and Anatomy & Cell Biology (MJB), Monash University, Australia

* To whom correspondence should be addressed. E-mail: azulli{at}unimelb.edu.au.

Submitted on July 14, 2005
Accepted on 19 July 2005


  Abstract
Evidence suggests that the angiotensin type 2 receptor (AT2R) and angiotensin converting enzyme 2 (ACE2) play a protective role in atherogenesis. These factors have not been identified in rabbit atherosclerotic plaques. Aim: To localize ACE2 and AT2R in rabbit atherosclerotic tissues, and determine which cell types express these factors. Methods: New Zealand White rabbits were fed either a control diet or a 0.5% cholesterol diet (n=8/group) for 12 weeks. Paraffin-fixed thoracic aorta were serially sectioned and processed for immunohistochemsity using commercially available antibodies to ACE2, AT2R, RAM 11 (to identify macrophages) and {alpha} smooth muscle cell actin ({alpha}SMC) to identify smooth muscle cells and myofibroblasts. Results: AT2R immunoreactivity, but not ACE2 immunoreactivity, was clearly present in endothelia overlying normal wall. However both AT2R and ACE2 immunoreactivity were clearly present in endothelia overlying neo-intima formation and atherosclerotic plaques. Within plaques, both AT2R and ACE2 immunoreactivity were observed in macrophages and {alpha}SMC actin positive cells. Examination of serial sections showed that the majority of cells were both ACE2 and AT2R positive. Conclusions: Macrophages and {alpha}SMC actin positive cells produce ACE2 and the AT2R in atherosclerotic plaques. Determining a role for these factors in the control of atherosclerosis will require additional studies.

Key Words: atherosclerosis, ACE2, AT2 receptors, immunohistochemistry


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