Journal of Histochemistry and Cytochemistry Priciples for Free Access to Science
  Search:   
    >> Advanced Search

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
JHC exPRESS: First Published March 20, 2006. doi:10.1369/jhc.5C6895.2006
Copyright © Histochemical Society, Inc.

A more recent version of this article appeared on August 1, 2006.
This Article
Right arrow exPRESS PDF
Right arrow All Versions of this Article:
jhc.5C6895.2006v1
54/8/849    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bencsik, A. A.
Right arrow Articles by Moussa, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bencsik, A. A.
Right arrow Articles by Moussa, A.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Rapid Communications

Amplified Immunohistochemical Detection of PrPsc in Animal Transmissible Spongiform Encephalopathies Using Streptomycin

Anna A. Bencsik 1*, Anthony W. Coleman 1, Sabine O.S. Debeer 1, Hervé Perron 1 and Aly Moussa 1

1 Agence Française de Sécurité Sanitaire des Aliments, Laboratoire d’Etudes et de Recherches en Pathologie Bovine et Hygiène des Viandes, Unité ATNC, Lyon, France (AAB,SOSD,AM); Institut de Biologie et de Chimie des Protéines, University Lyon 1, Lyon, France (AWC); and BioMérieux, R&D Immunoassay and Proteomics Department, Marcy L’Etoile, France (HP)

* To whom correspondence should be addressed. E-mail: a.bencsik{at}lyon.afssa.fr.

Submitted on December 10, 2005
Accepted on 8 March 2006


  Abstract
Due to its sensitivity, immunohistochemistry (IHC) of abnormal prion protein (PrPsc) is used to study experimental and natural cases of transmissible spongiform encephalopathies (TSEs), such as Creutzfeldt-Jakob disease in humans or scrapie and bovine spongiform encephalopathy (BSE) in animals. The limits of detection are particularly critical when PrPsc IHC is used for diagnostic purposes. In this article is described, for the first time, the use of streptomycin sulfate in IHC, providing a novel, original and easy way to amplify specifically PrPsc immunohistochemical detection in natural cases of BSE and scrapie, as well as in experimental TSEs in mice models using two different PrP antibodies.

Key Words: amplification, BSE, diagnosis, immunohistochemistry, prion, scrapie, streptomycin


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?





Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 2006