Immunohistochemical Localization of Phosphodiesterase 10A (PDE10A) in Multiple Mammalian Species

Timothy M. Coskran ^1*, Daniel Morton ¹, Frank S. Menniti ¹, Wendy O. Adamowicz ¹, Robin J. Kleiman ¹, Anne M. Ryan ¹, Christine A. Strick ¹, Christopher J. Schmidt ¹ and Diane T. Stephenson ¹

¹ Departments of Safety Sciences (TMC,DM,AMR,DTS) and CNS Discovery (FSM,WOA,RJK,CJS,CAS), Pfizer Global Research and Development, Groton, Connecticut

^* To whom correspondence should be addressed. E-mail: Timothy.M.Coskran{at}pfizer.com.

Submitted on January 20, 2006
Accepted on 27 June 2006

	Abstract

A monoclonal antibody directed against the amino terminal of rat PDE10A was used to localize PDE10A in multiple CNS and peripheral tissues from mouse, rat, dog, cynomolgus macaque and human. PDE10A immunoreactivity is strongly expressed in the CNS of these species with limited expression in peripheral tissues. Within the brain, strong immunoreactivity is present in both neuronal cell bodies and neuropil of the striatum, in striatonigral and striatopallidal white matter tracks, and in the substantia nigra and globus pallidus. Outside of the brain, PDE10A immunoreactivity is less intense and the distribution is limited to few tissues, such as the testis, epididymal sperm, and enteric ganglia. These data demonstrate that PDE10A is an evolutionarily conserved phosphodiesterase that is highly expressed in the brain, but with restricted distribution in the periphery in multiple mammalian species.

Key Words: PDE10A, phosphodiesterase, immunohistochemistry, localization, striatum, testis, enteric ganglia, nuclear

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