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JHC exPRESS: First Published June 26, 2006. doi:10.1369/jhc.6A6979.2006
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A more recent version of this article appeared on October 1, 2006.
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HuD Distribution Changes in Response to Heat-Shock, but Not Neurotrophic Stimulation

Richard W. Burry 1* and Catherine L. Smith 1

1 Department of Neuroscience, College of Medicine, The Ohio State University, Columbus, Ohio

* To whom correspondence should be addressed. E-mail: burry.1{at}osu.edu.

Submitted on March 28, 2006
Accepted on 5 June 2006


  Abstract
Cellular stress leads to a change in distribution of RNA-binding proteins. HuR, a member of the ELAV/Hu family of RNA binding proteins, is nuclear in distribution and following heat-shock is found in large cytoplasmic stress granules where translation is inhibited. HuD, another an ELAV/Hu RNA binding protein, stabilizes the GAP-43 mRNA in response to NGF stimulation in PC12 cells. We were interested in determining the nuclear distribution of HuD and determining if neurotrophic stimulation induced changes in the distribution of HuD. In PC12 cells, we found that HuR translocates from the nucleus to the cytoplasm in response to heat shock as expected. In response to heat-shock, HuD forms large cytoplasmic stress granules, consistent with a role for HuD in the cessation of translation. In unstimulated cells, HuD is distributed in small granules in the cytoplasm and is consistently present at low levels in the nucleus. Stimulation of PC12 cells with NGF induces neuronal differentiation including outgrowth of neurites, and increased levels of GAP-43 protein, whereas HuD remains localized in small cytoplasm granules and is still present in the nucleus. These results suggest that following neurotrophic stimulation, the lack of changes in HuD distribution are due to continued steady-state of HuD nuclear shuttling in PC12 cells, or that HuD is not normally shuttled from the nucleus in response to NGF..

Key Words: HuD, HuR, GAP-43, nuclear/cytoplasmic shuttling


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Home page
 Mol. Biol. CellHome page
H. Zhu, R. A. Hasman, V. A. Barron, G. Luo, and H. Lou
A Nuclear Function of Hu Proteins as Neuron-specific Alternative RNA Processing Regulators
Mol. Biol. Cell, December 1, 2006; 17(12): 5105 - 5114.
[Abstract] [Full Text] [PDF]



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