Extracellular Matrix-Mediated Tissue Remodeling Following Axial Movement of Teeth

Xianghong Luan ¹, Yoshihiro Ito ¹, Sean Holliday ¹, Cameron Walker ¹, Jon Daniel ¹, Therese M. Galang ¹, Tadayoshi Fukui ¹, Akira Yamane ¹, Ellen Begole ¹, Carla Evans ¹ and Thomas G.H. Diekwisch ^1*

¹ Department of Oral Biology (XL,YI,SH,CW,JD,TMG,TGHD) and Department of Orthodontics (XL,YI,SH,TMG,EB,CE,TGHD), UIC College of Dentistry, Chicago, Illinois, and Department of Pharmacology, Tsurumi University School of Dental Medicine, Yokohama, Japan (TF,AY)

^* To whom correspondence should be addressed. E-mail: E-mail: tomdkw{at}uic.edu.

Submitted on May 14, 2006
Accepted on 14 September 2006

	Abstract

Tooth eruption is a multifactorial process that involves the movement of existing tissues and the formation of new tissues coordinated by a complex set of genetic events. We have used the model of the un-opposed rodent molar to study morphologic and genetic mechanisms involved in axial movement of teeth. Following extraction of opposing upper molars, lower molars super-erupted by 0.13 mm. Labeled tissue sections revealed significant amounts of new bone and cementum apposition at the root apex of the un-opposed side following super-eruption for 12 days. Newly apposited cementum and alveolar bone layers were approximately three-fold thicker in the experimental versus the control group while periodontal ligament width was maintained. TRAP-staining indicated bone resorption at the mesial alveolar walls of un-opposed molars and provided in tandem with new bone formation at the distal alveolar walls an explanation for the distal drift of molars in this model. Microarray analysis and semiquantitative RT-PCR demonstrated a significant increase in collagen I, integrin 5, and SPARC gene expression as revealed by comparison between the un-opposed molar group and the control group. Immunohistochemical verification revealed increased levels of integrin 5 and SPARC labeling in the periodontal ligament of the un-opposed molar. Together, our findings suggested that posteruptive axial movement of teeth was accomplished by significant formation of new root cementum and alveolar bone at the root apex in tandem with upregulation of collagen I, integrin 5, and SPARC gene expression.
CiteULike    Complore    Connotea    Del.icio.us    Digg    Reddit    Technorati    Twitter    What's this?

This article has been cited by other articles:

				S. Lal, B. Cheng, S. Kaplan, B. Softness, E. Greenberg, R. S. Goland, E. Lalla, and I. B. Lamster Accelerated Tooth Eruption in Children With Diabetes Mellitus Pediatrics, May 1, 2008; 121(5): e1139 - e1143. [Abstract] [Full Text] [PDF]

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact

The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 2006