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JHC exPRESS: First Published October 16, 2006. doi:10.1369/jhc.6A7050.2006
Journal of Histochemistry and Cytochemistry
Copyright © 2006 Segret et al.
A more recent version of this article appeared on February 1, 2007.
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Articles |
Structural Localization and Expression of CXCL12 and CXCR4 in Rat Heart and Isolated Cardiac Myocytes
1 CNRS-UMR 8162, Université Paris XI Sud, Hôpital Marie-Lannelongue, Le Plessis-Robinson, France (AS,CR-M,JF,ED,AL,J-FR); INSERM U581, Université Paris-XII, Hôpital Henri Mondor, Créteil, France (CP); and Department of Ophthalmology 5, XV-XX Hospital, Paris, France (M-AC)
* To whom correspondence should be addressed. E-mail: audrey.segret{at}ccml.u-psud.fr.
Submitted on July 4, 2006
Accepted on 27 September 2006
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Abstract |
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are present in the normal heart. However, little is known about CXCL12 protein expression and localization. We investigated the pattern of protein expression and the localization of both CXCR4 and CXCL12 in the heart, using isolated cardiomyocytes and a rat myocardial infarction model. Western blots showed that cardiomyocytes contained a specific 67 kDa CXCR4 isoform and a 12 kDa CXCL12 isoform. Confocal and electron microscopy clearly showed that CXCR4 was present at the plasmalemma and CXCL12 in continuity of the Z-line, in the proximal part of T-tubules. In conclusion, we provide the first description of the expression and fine localization of CXCR4 and CXCL12 proteins in normal rat heart and cardiomyocytes. These results suggest that the CXCL12/CXCR4 axis may be involved in cardiomyocyte calcium homeostasis regulation. Our work and the well known chemoattraction properties of the CXCL12/CXCR4 axis highlight the importance of deciphering the function of this axis in both normal and pathological hearts.
Key Words: chemokine, CXCL12, CXCR4, heart, myocyte, T-tubules
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