Journal of Histochemistry and Cytochemistry Priciples for Free Access to Science
  Search:   
    >> Advanced Search

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
JHC exPRESS: First Published December 1, 2006. doi:10.1369/jhc.6A7083.2006
Copyright © Histochemical Society, Inc.

A more recent version of this article appeared on March 1, 2007.
This Article
Right arrow exPRESS PDF
Right arrow All Versions of this Article:
jhc.6A7083.2006v1
55/3/275    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Banas, M. C.
Right arrow Articles by Alpers, C. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Banas, M. C.
Right arrow Articles by Alpers, C. E.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

Articles

Localization of TGF-beta Signaling Intermediates Smad2, -3, -4, and -7 in Developing and Mature Human and Mouse Kidney

Miriam C. Banas 1*, W. Tony Parks 1, Kelly L. Hudkins 1, Bernhard Banas 1, Matthew Holdren 1, Masayuki Iyoda 1, Tomasz A. Wietecha 1, Jolanta Kowalewska 1, Gang Liu 1 and Charles E. Alpers 1

1 Department of Pathology, University of Washington, Seattle, Washington (MCB,WTP,KLH,MH,MI,TAW,JK,GL,CEA), and Klinik und Poliklinik für Innere Medizin II, University of Regensburg, Regensburg, Germany (MCB,BB)

* To whom correspondence should be addressed. E-mail: banas{at}gmx.de .

Submitted on August 18, 2006
Accepted on 13 November 2006


  Abstract
Smad proteins are signaling intermediates of the TGF-{beta} superfamily, which are involved in a range of biological activities including development and immune responses. We studied the expression of TGF-{beta}-receptor activated Smads (Smad2 and Smad3), the common partner Smad (Smad4), an inhibitory Smad (Smad7) and the activated (phosphorylated) Smad2 (pSmad2) in developing and adult kidneys of humans and mice. These studies demonstrate associated expression of these Smads in multiple renal cell types in all developmental stages and in mature non-diseased kidneys. Smad expression is in general most widespread at the earliest stages of nephron development and diminishes as components of the nephrons become more differentiated. Remarkable was the paucity of Smad expression in mesangial cells in contrast to widespread expression of these Smads in glomerular visceral epithelial cells in both developing and mature kidneys. Divergent and less extensive expression of Smad4, compared with other Smad proteins, was also demonstrated in tubules of human kidneys. Based on the observed expression patterns, these findings demonstrate for the first time expression of the TGF-{beta}-receptor activated Smad2 and Smad3, the common mediator Smad4 and the inhibitory Smad7 in the developing human fetal kidney, extending observations previously made in rodent systems to humans.

Key Words: Smads, TGF beta, renal pathology, kidney development, cell signaling, immunohistochemistry


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 J. Am. Soc. Nephrol.Home page
M. C. Banas, B. Banas, K. L. Hudkins, T. A. Wietecha, M. Iyoda, E. Bock, P. Hauser, J. W. Pippin, S. J. Shankland, K. D. Smith, et al.
TLR4 Links Podocytes with the Innate Immune System to Mediate Glomerular Injury
J. Am. Soc. Nephrol., April 1, 2008; 19(4): 704 - 713.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Soc. Nephrol.Home page
S. Taneda, K. L. Hudkins, A. S. Muhlfeld, J. Kowalewska, J. W. Pippin, S. J. Shankland, and C. E. Alpers
Protease Nexin-1, tPA, and PAI-1 are Upregulated in Cryoglobulinemic Membranoproliferative Glomerulonephritis
J. Am. Soc. Nephrol., February 1, 2008; 19(2): 243 - 251.
[Full Text] [PDF]



Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 2006