Expression of Leptin and Adiponectin in the Rat Oviduct

Marta Archanco ^1*, Javier Gómez-Ambrosi ¹, Manuel Tena-Sempere ¹, Gema Frühbeck ¹ and María A. Burrell ¹

¹ Department of Histology and Pathology (MA,MAB) and Metabolic Research Laboratory (JG-A,GF), University of Navarra, Pamplona, Spain; Department of Endocrinology, Clínica Universitaria, Pamplona, Spain (GF); and Department of Cell Biology, Physiology and Immunology, University of Córdoba, Córdoba, Spain (MT-S)

^* To whom correspondence should be addressed. E-mail: marclab{at}alumni.unav.es.

Submitted on October 11, 2006
Accepted on 15 May 2007

	Abstract

In mammals, the oviduct is an important source of factors which play key roles in reproductive and developmental events. The major components of oviduct fluid are oviduct-specific glycoproteins, but other proteins are synthesized and secreted by the oviduct epithelium. Leptin and adiponectin are two hormones, originally identified in adipocytes, that play a critical role not only in the control of energy balance and metabolism, but also in diverse functions such as reproduction. This study investigates the presence and distribution of leptin and adiponectin in the rat oviduct through a combination of immunohistochemistry (IHC) and Reverse Transcription Polymerase Chain Reaction (RT-PCR) techniques. Using both techniques, it has been detected that the oviduct of cycling rats expresses leptin and adiponectin. Immunoreactivity for both adipokines appears in the apical region of the secretory epithelial cells, only in the isthmus and ampulla. The immunostain is stronger in the isthmus and changes throughout the estrous cycle in the ampulla, increasing from proestrous to estrous. The results presented here are a further contribution to the identification of leptin and adiponectin produced and secreted by the oviduct epithelium, which must be taken into account for a better understanding of the reproductive events that take place in this organ.

Key Words: leptin, adiponectin, adipokines, rat oviduct, estrous cycle, secretory cells, oocyte, immunohistochemistry, RT-PCR

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