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JHC exPRESS: First Published February 20, 2007. doi:10.1369/jhc.6A7130.2007
Copyright © Histochemical Society, Inc.

A more recent version of this article appeared on June 1, 2007.
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Alteration in Renal Organic Anion Transporter 1 After Ischemia/Reperfusion in Cadaveric Renal Allografts

Osun Kwon 1*, Seok-Min Hong 1 and Kristina Blouch 1

1 Division of Nephrology, Department of Medicine, Indiana University School of Medicine, Indianapolis, Indiana (OK,S-MH), and Division of Nephrology, Department of Medicine, Stanford University School of Medicine, Stanford, California (KB)

* To whom correspondence should be addressed. E-mail: okwon{at}psu.edu.

Submitted on October 26, 2006
Accepted on 23 January 2007


  Abstract
We have previously shown that postischemic injury to renal allografts results in profound impairment of p-aminohippuric acid (PAH) extraction. To elucidate the cellular integrity of the human organic anion transporter 1 (hOAT1) in postischemic acute renal failure (ARF), immunohistochemical analysis of hOAT1 was performed in cadaveric renal allografts, using confocal microscopy for three-dimensional reconstruction of serial optical images. Biopsy samples were obtained from 10 cadaveric renal allografts an hour after reperfusion during transplant operation. Control tissues were obtained from four living donors of healthy kidneys immediately before an arterial clamp was applied to the renal artery. Control tissues demonstrated hOAT1 distributed to basolateral membrane of proximal tubule cells. In contrast, maldistribution of hOAT1 to cytoplasm and/or diminution of the protein was noted in cadaveric allografts. The characteristics of maldistribution were variable: disappearance of lateral distribution, diffuse cytoplasmic aggregates, apical cytoplasmic aggregates, and disappearance of the staining. In addition, iothalamate and PAH clearances were performed on post-transplant day 3-7 in 18 recipients of a cadaveric renal allograft. PAH clearance was depressed < 250 ml/min in all but three subjects. We conclude that reperfused, transplanted kidneys exhibit maldistribution of hOAT1 in proximal tubule cells, resulting in impairment of PAH clearance. This manuscript contains online supplemental material at http://www.jhc.org. Please visit this article online to view these materials.

Key Words: postischemic acute renal failure, renal tubular secretion, hOAT1, confocal microscopy


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