Journal of Histochemistry and Cytochemistry
  Search:   
    >> Advanced Search

Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact

JHC exPRESS: First Published March 19, 2007. doi:10.1369/jhc.6A7132.2007
Journal of Histochemistry and Cytochemistry
Copyright © 2007 Khobarekar et al.

A more recent version of this article appeared on July 1, 2007.
This Article
Right arrow exPRESS PDF
Right arrow All Versions of this Article:
jhc.6A7132.2007v1
55/7/753    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Khobarekar, B. G.
Right arrow Articles by Bandivdekar, A. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Khobarekar, B. G.
Right arrow Articles by Bandivdekar, A. H.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Articles

Studies on the Expression of 80kDa Human Sperm Antigen (80kDaHSA) in Rat Testis and Epididymis

Bharati G. Khobarekar 1, Vandana J. Vernekar 1, E. Prabagaran 1, Vijaya P. Raghavan 1 and Atmaram H. Bandivdekar 1*

1 National Institute for Research in Reproductive Health (ICMR), Mumbai, India

* To whom correspondence should be addressed. E-mail: batmaram{at}gmail.com.

Submitted on October 27, 2006
Accepted on 6 March 2007


  Abstract
80kDa Human Sperm Antigen (80kDaHSA) demonstrated to be the promising candidate for development of an antifertility vaccine as it is sperm specific, conserved and immunogenic protein. The present study demonstrates the androgen-regulated expression of 80kDa HSA in testis and epididymis of rat by immunohistochemistry (IHC) using its specific antibodies. The developmental expression of 80kDaHSA was investigated on day 10, 20, 40, 60 and 90 of age in the testis and epididymis by IHC and the relative staining intensity was estimated by image analysis using BIOVIS software. On day 10 and 20 of age no significant staining was observed in the testis and epididymis while it gradually increased from day 40 onwards. The highest staining was seen on day 90 of age in both testis and epididymis. Gradual increase in expression of 80kDaHSA after day 40 of age suggests that it is possibly regulated by androgen. To study the androgen-regulated expression of 80kDaHSA the adult male rats were treated with 75mg/kg body weight of Ethylene Dimethane Sulfonate (EDS), which selectively destroys leydig cells and thus induces complete androgen withdrawal. It was observed that the intensity of staining decreased following EDS treatment in rat testis as well as epididymis and it was regained after supplementation with dihydrotestosterone (DHT). Increased expression during sexual maturation at the time of testosterone surge and its regulation by antiandrogen/androgen treatment suggest androgen dependent expression of 80kDa HSA in testis and epididymis of rat.

Key Words: sperm antigen, developmental expression, androgen, antiandrogen, immunohistochemistry, immunocontraception


Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?


This article has been cited by other articles:


Home page
 J AndrolHome page
E. Prabagaran, U. C. Hegde, S. B. Moodbidri, A. H. Bandivdekar, and V. P. Raghavan
Postnatal Expression and Androgen Regulation of HOXBES2 Homeoprotein in Rat Epididymis
J Androl, September 1, 2007; 28(5): 755 - 771.
[Abstract] [Full Text] [PDF]



Guidelines | Subscriptions | About | exPRESS - Current - Archive | Business Information | Contact
The Journal of Histochemistry & Cytochemistry is owned, published, and licensed by The Histochemical Society © 2007

 
Purchase HCS Short Course Manual on HCS site