The Actin Binding Proteins Coronin-1a and IBA-1 Are Effective Microglial Markers for Immunohistochemistry

Zeshan Ahmed ¹, Gerry Shaw ¹, Ved P. Sharma ¹, Cui Yang ¹, Eileen McGowan ¹ and Dennis W. Dickson ^1*

¹ Department of Neuroscience, Mayo Clinic College of Medicine, Jacksonville, Florida (ZA,EM,DWD), and Department of Neuroscience, McKnight Brain Institute, University of Florida College of Medicine (GS,CY) and Department of Chemical Engineering (VPS), University of Florida, Gainesville, Florida

^* To whom correspondence should be addressed. E-mail: dickson.dennis{at}mayo.edu.

Submitted on November 22, 2006
Accepted on 15 February 2007

	Abstract

This study identifies the actin binding protein coronin-1a as a novel and effective immunohistochemical marker for microglia in both cell cultures and in formaldehyde-fixed, paraffin-embedded tissue. Antibodies to coronin-1a effectively immunostained microglia in human, monkey, horse, rat and mouse tissues, even in tissues that had been stored for long periods of time. The identity of coronin-1a immunoreactive cells as microglia was confirmed using double immunolabeling with cell type-specific markers as well as by morphologic features and the distribution of immunoreactive cells. These properties are shared by another actin binding protein, IBA-1. Unlike IBA-1, coronin-1a immunoreactivity was also detected in lymphocytes and certain other hematopoietic cells. The results indicate that both coronin-1a and IBA-1 are robust markers for microglia that can be used in routinely processed tissue of humans and animals. Since both coronin-1a and IBA-1 are actin binding proteins that play a role in rearrangement of the membrane cytoskeleton, it suggests that these proteins be critical to dynamic properties of microglia.

Key Words: coronin-1a, IBA-1, microglia, immunohistochemistry

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