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JHC exPRESS: First Published November 12, 2007. doi:10.1369/jhc.7A7365.2007
Journal of Histochemistry and Cytochemistry
Copyright © 2007 Taylor-Fishwick et al.

A more recent version of this article appeared on February 1, 2008.
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Articles

Pancreatic Islet Immunoreactivity to the Reg Protein INGAP

David A. Taylor-Fishwick 1*, Angela Bowman 1, MariCarmen Korngiebel-Rosique 1 and Aaron I. Vinik 1

1 Departments of Medicine, Microbiology and Molecular Cell Biology (DAT-F) and Anatomy and Pathology (AB,MK-R,AIV), Eastern Virginia Medical School, Norfolk, Virginia

* To whom correspondence should be addressed. E-mail: Taylord{at}evms.edu.

Submitted on October 10, 2007
Accepted on 25 October 2007


  Abstract
The Reg-related protein family member INGAP (islet neogenesis associated protein) is a pleiotropic factor, enhancing islet neogenesis, neurite growth, beta cell protection and beta cell function. Using an antibody to the N-termini of INGAP, we have identified immunoreactivity to INGAP localized to the pancreatic endocrine cells in mouse. INGAP-immunoreactive and insulin-immunoreactive cells are mutually exclusive; with INGAP-immunoreactive cells being preserved after STZ-mediated destruction of beta cells. Glucagon-immunoreactive cells and INGAP-immunoreactive cells co-localize, although respective antigen expression occurs in different intra-cellular locations. These data suggest that INGAP-immunoreactive cells include alpha cells, however, the detection of single INGAP-immunoreactive/glucagon-negative cells indicate this may not be exclusive. In addition to mouse, the detection of islet endocrine cells that were INGAP-immunoreactive/glucagon-immunoreactive/insulin-negative were also observed in islets from human, monkey and rat. These findings reveal that INGAP and/or related group 3 Reg proteins have a conserved expression in the pancreatic islet.

Key Words: islet, glucagon, islet neogenesis associated protein


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