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JHC exPRESS: First Published December 23, 2007. doi:10.1369/jhc.7A7366.2007
Copyright © Histochemical Society, Inc.

A more recent version of this article appeared on May 1, 2008.
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Expression of Aquaporin 9 in Rat Liver and Efferent Ducts of the Male Reproductive System After Neonatal Diethylstilbestrol Exposure

Anja Wellejus 1*, Henrik E. Jensen 1, Steffen Loft 1 and Thomas E. Jonassen 1

1 Department of Pharmacology, The Panum Institute (AW,TEJ) and Department of Occupational and Environmental Health (AW,SL), University of Copenhagen, Copenhagen, Denmark, and Department of Veterinary Pathobiology, Faculty of Life Sciences, University of Copenhagen, Frederiksberg, Denmark (HEJ)

* To whom correspondence should be addressed. E-mail: anwe{at}pubhealth.ku.dk.

Submitted on August 7, 2007
Accepted on 5 December 2007


  Abstract
Aquaporins (AQP) have important solute transport functions in many tissues, including the epididymal efferent ducts (ED) and in the liver. We investigated the effect of neonatal exposure to of diethylstilbestrol (DES) on AQP9 expressions in the ED and the liver of rats. DES was administered from day 2 to day 20 postnatally at a dose of 4,8 µg/day and AQP9 protein and mRNA were measured by immuno blotting and real time-PCR, respectively along with immunohistochemistry. DES caused hepatic downregulation of AQP9 at both the protein and mRNA level, however decreased AQP9 labelling was only observed in the periportal zone. In the ED, AQP9 protein expression was increased by 300% in the DES treated animals which could be ascribed to a widening of the ED lumen, whereas no difference was observed in AQP9 mRNA expression. The immunohistochemical findings revealed that AQP9 expression was confined to the epithelial cells of the ED. In conclusion, neonatal DES exposure appear to upregulate AQP9 channels in the ED in male rats, whereas a downregulation in the hepatic expression was observed, particularly in the periacinous area.

Key Words: epididymal efferent ducts, estrogen, liver, aquaporin 9, diethylstilbestrol


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