Editorial Office Contact Information

Editorial Office

Dr. Kevin A. Roth, Editor-in-Chief

University of Alabama at Birmingham

Department of Pathology, Division of Neuropathology

Phone, Fax, and Email:

Phone: 206-616-5894

FAX: 206-616-5842

E-mail: johc{at}u.washington.edu

Print Correspondence (US Mail, Surface Mail, Airmail) Address:

Dr. Denis G. Baskin, Executive Editor

The Journal of Histochemistry and Cytochemistry

Department of Biological Structure, Box 357420

University of Washington School of Medicine

Seattle, WA 98195-7420

USA

Express carrier service (Express Mail, UPS, DHL, TNT, FedEx) address:

Dr. Denis G. Baskin, Executive Editor

The Journal of Histochemistry and Cytochemistry

University of Washington

1914 N. 34th St., Suite 400

Seattle, WA 98103

USA

Authors are invited to submit manuscripts for publication as Articles (full-length research reports), Reviews, Perspectives, and Letters to the Editor.

Before submitting your manuscript to JHC, authors must read our Editorial Policies. These describe our requirements regarding human and animal research, materials and methods disclosure, and prior publication. Information regarding our expectations of reviewers and editors and our Review Process are also available on this page.

Manuscript submissions are done via JHC Bench>Press at <http://submit.jhc.org>. If you are new to the system, the author who is submitting the paper will need to create a profile. Click on the create a new account link, and you will be guided through the process of making your new profile. Once this is completed, return to the main page and log in with your username and password. The option to submit a manuscript should be available at the top of the screen. JHC Bench>Press will give authors instructions on submitting their paper.

If you have questions regarding manuscript submission, feel free to contact us.

1. The Manuscript Title.
   
2. The Author(s) name(s) (identify the corresponding author, with complete address, telephone, FAX, and e-mail information).
   
3. The Type of manuscript (Article, Review, Perspective, Letter to the Editor)
   
4. A Brief statement of the significance of the paper.
   
5. A Statement that all of the authors have directly participated in the planning, execution, or analysis of the study and resulting paper, and have read and approved the version submitted.
   
6. A Statement that the authors agree to pay the cost of color illustrations. (Note: The first figure of color is free. Each additional figure is $300.)
   
7. (Optional) The contact information of any suggested reviewers (names, addresses, and e-mail).
   

Click here to view and print an HTML version of the form.

Alternatively, follow this link to a PDF file of the form.

A manuscript will not be reviewed, and no final decision will be rendered, without the completed and signed form.

Manuscript File
The manuscript file should be in Microsoft Word compatible format. We can accommodate other software, but staying in Word will give the best results. Follow these specifications when submitting the manuscript file:

• Do not attempt to produce a typeset look or use any special formatting. This could interfere with the production process.
• Do not use the 'Insert Symbol' function in Word (or in other word processors). For Greek symbols such as mu and alpha, please use the Symbol Font, which is available as a standard font with most word processors.
• Use the word processor's superscript or subscript functions. Do not attempt to achieve this effect by varying point size or positioning.
• Special Note for users of 'Track Changes' or similar features: If you use the Track Changes feature of Microsoft Word, or another word processor, before submitting your manuscript document file you must accept or reject all of your changes and turn the change tracking option off for the manuscript document file. If our Editorial Office is prompted by software to accept or reject alterations when your file is handled, we will accept all changes, and the authors will be responsible for any errors this generates.

Page and Content Layout
Manuscript documents should be formatted as follows:

• Double-spaced
• 2.5 cm (1 inch) margins
• Left-justification
• Page numbers at the bottom of each page; centered or right-justified
• 12 point font; restrict fonts to Times New Roman, Times, or Arial throughout the manuscript (Use of other fonts is not recommended and could result in problems with converting your manuscript for review.)
• Avoid boldface, underlining, or italics anywhere in the manuscript

Other formatting notes: We do not publish Footnotes or Appendicies. Please do not embed figures into the manuscript. They must be provided as separate files for each figure.

The layout of your manuscript's sections should be as follows:
• Page 1. Running headline: all capitals, no more than 48 characters and spaces.
• Page 2. Title page: Complete title; first name, middle initial, last name of each author; where work was done (use authors' initials in parentheses if necessary); mailing address, phone, FAX, and e-mail of the author designated for correspondence and reprint requests (if different from the first author).
• Page 3. Abstract: not more than 200 words, followed by 3 to 10 key words.
• Beginning on page 4: Introduction, Materials and Methods, Results, Discussion, Acknowledgments, Literature Cited, Figure Legends, Tables and Table Legends.

Begin each section on a new page. Individual sections, including figure legends, must all be contained in the same single document file. Do not send separate files for any section of the manuscript. Pasting any section, or any information from the manuscript file, into the review system is not a substitute for providing it in the manuscript document file. If a manuscript lacks any of these sections, it will delayed for review until they are provided by the authors.

Abbreviations and Style
Customary abbreviations in wide use need not be defined in text (e.g., DNA, ATP). Define other abbreviations the first time that they are used. Refer to the Journal of Biological Chemistry for recommended abbreviations for biological compounds, Chemical Abstracts for names of chemical compounds, Conn's Biological Stains (10th Edition, RW Horobin and JA Kiernan (eds.), BIOS Scientific Publishers) for nomenclature, and the CBE Style Manual (1994, 6th ed, Chicago, Council of Biology Editors; a 7th edition is being written) for scientific abbreviations. Use SI units only. The Journal does not print the degree symbol before temperature symbols. All temperatures are printed as follows: 78C, 32F, 276K.

Materials and Methods
Give the manufacturer's name and location (city and state for US, city and country for outside the US) in parentheses for reagents and instruments. Indicate the sources for all antibodies and nucleotide sequences. Any new antibodies must be characterized by biochemical methods such as Western blots. Controls for all histochemical methods must be reported. For studies that employ animals, indicate whether institutional or National Research Council guidelines were followed. If human subjects were used, indicate whether the procedures followed were in accordance with the Helsinki Declaration of 1975 or if they were approved by an institutional committee on human experimentation. Quantitative results must be presented as graphs or tables and supported by appropriate experimental design and statistical tests. Group data should be reported as means and standard errors, with statistical p-values and sample sizes.

Literature Cited
Reference only work published or in press, and give the name of the journal. Cite unpublished work as such in the text. Personal communications should be acknowledged in the text, and be accompanied by written permission. Abbreviations should conform to PubMed format. In the text, cite references by name and year, with earlier references listed first, and references with the same year listed alphabetically; eg. Lillie (1966), (Spicer et al. 1965; Graham and Karnovsky 1966). In Literature Cited, list references according to the Harvard Style (Name and Year). The Journal does not use Vancouver (numbered) citation format. Order references alphabetically by first author, and include all co-authors. When references have the same list of authors, order them chronologically, beginning with the earliest date of publication. When the same author has more than one publication in a year, use lower case letters (eg. 1989a, 1989b, etc.). Do not use periods after authors' initials, abbreviated journal titles, or at the end of the citation. Use inclusive page numbers. Examples:

Articles in Journals

Graham RC, Karnovsky MJ (1966) The early stages of absorption of injected horseradish peroxidase in the proximal tubules of the mouse kidney. Ultrastructural cytochemistry by a new technique. J Histochem Cytochem 14:291-302

Lillie RD (1966) Histochemical acylation of aldehydes produced by periodic acid. J Histochem Cytochem 14:529-537

Spicer SS, Leppi TJ, Stoward PJ (1965) Suggestions for a histochemical terminology for carbohydrate-rich tissue components. J Histochem Cytochem 13:599-603

Rothmann C, Cohen AM, Malik Z (In Press) Chromatin Condensation in Erythropoiesis Resolved by Multipixel Spectral Imaging: Differentiation Versus Apoptosis. J Histochem Cytochem


Books

Coons AH (1978) Fluorescent antibody methods. In Danielli JF, ed. General Cytochemical Methods. New York, Academic Press, 399-422

Sternberger LA (1979) Immunocytochemistry, 2nd ed. New York, Wiley


Special Note regarding Reference/Citation Managers
You may wish to use a citation or reference managers to assemble your references. Such programs generally use 'field codes' to track and arrange the citations in your manuscript. Unfortunately, field codes can (very unintentionally) result in deletions when the manuscript document file is used for publication. JHC requires that all field codes be removed from your manuscript text before it is submitted for publication. The following exerpt from Adept Scientific describes how to do this in Microsoft Word:


Many publishers require that you remove ALL field codes before submitting an electronic manuscript. Field codes are used in Microsoft Word to link your citations to your bibliographic references.

Make sure you remove hidden codes in a copy of your final manuscript. Please note that once you remove all hidden codes, you can no longer reformat or unformat the citations or bibliography so always make a copy of your document prior to removing any codes.
1. Make a copy of the final manuscript. From the File menu in Word, select the Save As command. Give the file a new name.
2. In the new file, go to the Edit menu and choose Select All.
3. Press Ctrl+Shift+F9 or Cmd+6 to unlink all fields. Your in-text citations and bibliography become regular text, without field codes or any hidden links.
If you want to do further editing or change citations in any way, make the changes to the original file. When you are ready to submit your manuscript, make another copy of the original file to unlink field codes.
Some citation managers have their own methods for removing field codes. Click here for information on how to remove field codes if you use EndNote.

Figure Legends
Figure legends must be placed after the Literature Cited. Manuscript document files lacking figure legends will not be reviewed. Do not duplicate material from the text or described in the methods in your figure legends. Indicate scale bar size if it is not indicated on the figure.

Tables
Each table must be appended to the end of the manuscript, after the Figure Legends, in either Word or Excel table format. Do not embed photographs or image files of tables. Legends or keys must accompany each table and should not be added to the Figure Legends. Tables should be numbered consecutively using Arabic numerals, and should include a brief title. Use footnotes (superscripted lower-case letters) to explain abbreviations, statistics, etc.

Figures - Updated!
Figure Guidelines can be found here, on a separate webpage (this link will open in a new web browser window). Please note: JHC now requires all color figures be submitted in RGB color mode! (See the Figure Guidelines for details.)

Supplemental Materials are images, movies, data sets, and other files that support and augment the content of a JHC manuscript, and they are intended for publication only in the Online version of JHC. These materials are referenced in your manuscript, and then accessed via the online Journal. Supplemental Materials generally fall into the following categories:

1. Extra Tables
2. Extra Figures
3. Datasets (eg. sequences, sequence alignments, GenBank Accession information)
4. Movies

• They must be submitted with your manuscript at the time of submission. Supplemental materials cannot be added to a manuscript after it has been accepted for publication, and are subject to review by the Editors of JHC.
• They must be accompanied by a complete set of legends, one legend for each supplemental item. The supplemental materials legend set must be appended to the end of the legends for your main figure set in the manuscript document file. (Large sets can also be accompanied by an introductory legend if needed.)
• They must be referenced in your manuscript body at least once.

Use the following when citing your Supplemental Data in the manuscript text:
Tables: Table ST1, ST2, ST3, etc.
Figures: Figure SF1, SF2A, SF2B-G, SF3, etc.
Datasets: Dataset SD1, SD2, SD3, etc.
Movies: Movie SM1, SM2, SM3, etc.

Examples of citing Online Supplemental Material: See online Supplementary Materials, Movie SM1 or An example of this is presented in online Supplemental Figure SF4.

Charges for Production of Supplemental Materials

• Storing supplemental materials on the JHC website is billed as follows:
  • Up to 5 Megabytes total: $115
  • More than 5 Megabytes, up to 10 Megabytes: $185
  • Each additional 5 Megabytes over 10 Megabytes: $100
• All charges related to alterations and storing of supplemental materials are due with your manuscript processing fee, at acceptance.
• Any alterations or corrections that must be made by the JHC Editorial Office to place your Supplemental Materials online will be charged at a rate of $100US per hour, and billed in 15 minute increments. Please be sure to format your materials correctly prior to submitting them for review and publication.

Supplemental Material Formats

Tables should be supplied in one of the following formats:
• Excel 2000 compatible spreadsheets
• Comma-delimited text file
• Tab-formatted Word 2000 compatible file
Images and figures should be supplied in one of the following formats:
• JPG file format
• TIFF file format
• Powerpoint slides
Any image file (continuous tone image, graph, chart, diagram, etc.) should be at least 200 dots per inch in resolution. Authors are encouraged to make sure that lineweight, labeling, and details on line art are legible when viewed at regular size on a computer monitor. If not, a higher resolution may be necessary, but images should be no more than 600 dots per inch. Submit your color supplemental image files in RGB color mode.

Movie files should be supplied in one of the following formats:
• AVI movie format
• Quicktime movie format
JHC will not perform file conversion services for any image or movie files. Please supply your image or movie files in the format intended for online publication.

Other Supplemental Materials Formats

• Databases should be presented as a series of Excel 2000 compatible tables.
• Reports derived from databases should be formatted in Word 2000 compatible format.
• Sequences should be presented in Word 2000 compatible files. Any sequences submitted to GenBank must have their accession numbers listed at the beginning of the sequence block. Colored sequence alignments should be presented in Word 2000 compatible Rich Text Format (rtf) or Post Script format (ps). FASTA or GenBank format can be used in layout of a sequence block, and ALN or MSF format can be used for Multiple Alignments.

Revised manuscripts should be resubmitted with adherence to the following guidelines:

1. The Letter of Transmittal must contain a point by point response to the reviewers' and editors' comments. Do not separate the Cover Letter and the Point by Point into two separate files. They should be contained in the same, single document file.

2. The figures should be production quality source image files. Do not submit low-resolution (less than 300 DPI at the size intended for publication) files to save time. If you encounter difficulties in submitting your production quality figures through the JHC Bench>Press, please email johc{at}u.washington.edu for information on obtaining a login and password for our local FTP server. This will allow you to use an FTP client (WS_FTP, Fetch, Internet Explorer) to upload your files directly to our offices. If you are submitting your figures on digital media, all figures must be supplied on Zip Disk, DVD, or CD in the appropriate file format (please see our figure guidelines for more details).

3. Figures must be the final format intended for publication and should have all sizing and labeling completed. Alterations in figure sizing, layout, and composition made by the Editorial Office will incur an additional cost. Figure alterations are charged at $100US/hour.

4. If the manuscript title has changed significantly at revision, a new License to Publish signed by the corresponding author must be supplied at the time of submission. If a new License to Publish is not submitted, no final decision will be rendered. If any authors have been removed from the paper, there should be a brief statement as to why in the Covering Letter, and a letter from the author(s) that were removed stating their approval of this action.

Once a manuscript has been Accedpted, the following will happen:

• An exPRESS PDF article will be produced and put into our queue for exPRESS Publication. Please see the exPRESS section for more details on exPRESS, JHC's ePublish-Ahead-of-Print service for authors.
• Your manuscript is copyedited, during which time you may be queried by our copy-editors for answers to questions. Authors have 48 hours to respond to these queries, after which the manuscript is sent to type-setting. Copy-editing is provided free of charge, but please see our fees section for information on charges that may be incurred if the author fails to respond to these queries (Author's Alterations).
• Your manuscript is typeset, and Authors' Pages (also known as Galley Proofs or Proofs) are produced in PDF format. A link to download the PDF proof is provided in an email, along with instructions for marking changes to your pages. Please see our fees section for information on charges that may be incurred when you correct your page-proofs (Author's Alterations).
• Your changes are sent to the publisher, who corrects the type-set article as needed.
• Your article is finalized in the JHC Offices, and assigned to an issue. Congratulations!
• JHC automatically deposits all of its articles into PubMed Central as per the NIH's Public Access policy. If you need to know your PubMed Central ID, it will appear in PubMed once your manuscript has been published. Please see Wake Forest University Health Sciences' instructions for locating your PMCID in PubMed once your article has been indexed.

No fees are charged to an author until their manuscript has been Accepted. Upon Acceptance, fees relating to production are due, and additional fees may be incurred over the course of production.

• Processing Fee (mandatory) - This fee is charged to offset the costs incurred by JHC to review and publish your article. For all manuscripts originally submitted prior to September 1, 2007, the processing fee is $350US. For all manuscripts originally submitted on or after September 1, 2007, the processing fee is $450US.
• Color Production Charge (mandatory) - For any manuscript containing more than 1 color figure, this fee is mandatory. The charge for color production is $300US per color figure, with your first color figure being free of charge. This charge is on a per-figure-file-basis, thus if a single figure spans more than one file, it will count as separate figures for the purposes of billing. If a figure intended for grayscale is submitted as a color figure, and sent to production as a color figure, authors will be billed for processing of the color figure.

The Processing Fee and Color Production Charge will be billed to the corresponding author unless an alterate billing address has been supplied. This address must be indicated in a response email when you receive your Accept email letter. Your manuscript will not be published until payment of all mandatory charges is received.


• Author's Alterations (incurred) - These are corrections or changes to text and figures requested by the author after the manuscript has been type-set (i.e. changes made in pages or page-proofs). Author's Alterations are billed as follows:
  • $30.00 US per change to any single word in text
  • $30.00 US per revision or replacement of a grayscale/black-and-white figure
  • $65.00 US per revision or replacement of a color figure
  
  Please note:
  • Corrections made in response to copyeditor and/or printer queries in typeset page proofs are free of charge. Changes specifically initiated and requested by the authors are charged as Author's Alterations.
  • Your manuscript will be scheduled for publication upon receipt of payment for all mandatory and incurred charges.

An Offprint Order Form will accompany the PDF Page Proofs. This order form should be filled out and returned along with your Page Proofs. Offprints cannot be ordered after your manuscript has been published.

Reprints can be ordered after publication via The Histochemical Society's eCommerce Store. These reprints are printed from high-resolution PDFs of the original, published manuscript. Use the eCommerce Store's Quote Request Form to request a pricing quote on the reprints. You do not need to be an author to order reprints of an article. The complete pricing sheet for reprints can be seen here. A PDF reader (such as Adobe Acrobat Reader) is needed to view the reprint order pricing.

The Journal of Histochemistry and Cytochemistry has begun exPRESS, a publish-ahead-of-print service for the rapid, on-line publication of manuscripts that are Accepted by JHC. In the event your paper is accepted, it will be published online ahead of its print publication date in raw, PDF form. The PDF will appear on-line exactly as it was submitted, with all of the figures and tables. All manuscripts accepted in JHC are exPRESS published. Supplemental Materials are not available with the exPRESS publication, they are added to the final published manuscript.

The title, authors' names, and abstract must be completely free of errors when the manuscript is submitted. Authors will not be queried for changes to these sections, nor any other part of their paper, if the manuscript is Accepted and sent for exPRESS publication. Changes and corrections can and will be made to the final version of the type-set paper that appears in print and on-line, but the PubMed Index entry is based on the first publication of a manuscript, which would be the on-line exPRESS publication. Carefully check your manuscript's title, authors' names, and abstract. No other corrections will be made at the time of exPRESS publication.

Reproduction of content previously published in the Journal of Histchemistry and Cytochemistry requires the permission of the Journal. To obtain permission, email the following information to johc{at}u.washington.edu:

• Complete mailing address, phone/fax number, and email address of the requesting author(s).
  
• Complete bibliographic information for the JHC article to be reproduced. Please list what will be reproduced. If you intend to modify the content, you must specify what will be altered and provide a photocopy example of the alterations.
  
• Complete bibliographic information (or purpose of use) for the article/book/publication the JHC content will appear in. Give an approximate publication date if possible.